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Macvector mutagenesis6/27/2023 ![]() ![]() An ABC transporter containing a forkhead-associated domain interacts with a serine-threonine protein kinase and is required for growth of Mycobacterium tuberculosis in mice. Mycobacterium tuberculosis cells growing in macrophages are filamentous and deficient in FtsZ rings. 2003 113:183–193.Ĭhauhan A., Madiraju M.V., Fol M., Lofton H., Maloney E., Reynolds R., Rajagopalan M. DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis. 1990 18:6069–6074.īardarov S., Bardarov S., Jr, Pavelka M.S., Jr, Sambandamurthy V., Larsen M., Tufariello J., Chan J., Hatfull G., Jacobs W.R., Jr Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. ![]() Ligation-independent cloning of PCR products (LIC-PCR) Nucleic Acids Res. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982).Aslanidis C., de Jong P.J. Genetics of actin-related sequences in tomato. Fatty acid composition of leaf lipids determined after combined digestion and fatty acid methyl ester formation from fresh tissue. Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica. Transformation and regeneration of Brassica rapa using Agrobacterium tumefaciens. Improved binary vectors for Agrobacterium-mediated plant transformation. Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Redesign of soluble fatty acid desaturases from plants for altered substrate specificity and double bond position. Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions. The catalytic activity of cytochrome P450cam towards styrene oxidation is increased by site-specific mutagenesis. Nickerson, D.P., Harford-Cross, C.F., Fulcher, S.R. The catalytic cysteine and histidine in the plant acyl-acyl carrier protein thioesterases. Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. Molecular dissection of the plant acyl-acyl carrier protein thioesterases. A synthetic oligonucleotide is made whose sequence matches the sequence of a region of the parent or wild type DNA except for a specific nucleotide. Site-directed mutagenesis is a powerful technique for introducing a mutation into a DNA sequence at a single known location. Setlow, J.K.) 111–133 (Plenum, New York 1996).įacciotti, M. An example using OR logic: site-directed mutagenesis. Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Characterization of acyl-ACP thioesterases of mangosteen ( Garcinia mangostana) seed and high levels of stearate production in transgenic canola. ![]() International News on Fats, Oils and Related Materials 7, 230–243 (1996). Trans fatty acids and their effects on lipoproteins in humans. Dietary fat, Trans fatty acids, and risk of coronary heart disease.
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